Single-cell CRISPR screening on mouse embryonic stem cells. Single-cell CRISPR screening on mouse embryonic stem cells

We performed CROP-seq experiments to interrogate genes that are critical for mouse embryonic stem cell pluripotency and differentiation Overall design: Examination of total 15 pluripotency-associated transcription factors and metabolic genes by perturbation

我们开展了CROP-seq实验，以探究对小鼠胚胎干细胞多能性与分化至关重要的基因。实验整体设计：通过基因扰动方式，共检测15个与多能性相关的转录因子及代谢基因。