miRNA expression in aging skin

Fibroblasts were isolated from healthy skin, donors were young, middlege and old. We used Affymetrix miRNA microarrays to detail the global expression of miRNAs. Primary fibroblasts were isolated from healthy skin, donnors were young, middlege and old and kept in culture for a short time in order to avoid scenescence.

本研究从健康皮肤中分离成纤维细胞（fibroblasts），供体涵盖青年、中年与老年群体。我们采用Affymetrix miRNA微阵列（miRNA microarrays）对microRNA（miRNA）的全局表达谱进行详细分析。另有一组原代成纤维细胞同样取自健康皮肤，供体同样分为青年、中年及老年，为避免细胞衰老（senescence），仅在体外进行短期培养。