Immunological memory diversity in the human upper airway. Immunological memory diversity in the human upper airway

Limited CITE-seq antibody panel and 5' gene expression profiling of human nasopharyngeal cells collected with flocked swabs, paired with TCR and BCR sequencing of NP lymphocytes. FACS-sorted SARS-CoV-2 antigen-specific memory B and T cells with 5' gene expression profiling and scBCR and scTCRseq. Overall design: 10x Genomics 5' immune profiling using BioLegend TotalSeq-C TBNK, hashtag antibodies, and SARS-CoV-2 labeled probes.

本数据集包含两类核心数据：一是采用植绒拭子采集的人鼻咽细胞的有限CITE-seq（Cellular Indexing of Transcriptomes and Epitopes by Sequencing）抗体组合分析与5'端基因表达谱分析，同步配套配对的鼻咽淋巴细胞T细胞受体（TCR）与B细胞受体（BCR）测序；二是经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）获取的SARS-CoV-2抗原特异性记忆B细胞与T细胞的5'端基因表达谱分析，以及单细胞BCR测序（scBCR）与单细胞TCR测序（scTCRseq）。实验整体设计：采用BioLegend公司的TotalSeq-C TBNK抗体组合、标签抗体及SARS-CoV-2标记探针，通过10x Genomics平台完成5'端免疫组库分析。