ICL0012 - Host response in human Calu-3 cells to infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1)

Differential expression was determined in Calu-3 cells between mock infected and infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1) at different times post infection. Purpose: To obtain samples for transcriptional analysis in triplicate using the VN1203 pathogenicity mutant NS1-trunc124. Overview of Experiment: . Time Points: 0, 3, 7, 12, 18 and 24 hrs post infection. . Done in triplicate. . Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates. . Time-matched mocks were done in triplicate from the same cell stock as the rest of the samples. . Culture medium (the same as what the virus stock is in) was used for the mock infections. Calu-3 cells were infected with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or mock infected and samples were collected at 0, 3, 7, 12, 18 and 24 hpi. Calu-3 cells were infected with WT: A/Vietnam/1203/2004 (H5N1) and samples were collected at 7 and 24 hpi. There are 3 mock (2 included here) and 3 infected replicates for each time point. Expression profiles were determined.

本研究在Calu-3细胞中检测了模拟感染（mock infection）、感染NS1截短124株(NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1))以及感染野生型(Wild Type, WT: A/Vietnam/1203/2004 (H5N1))后不同时间点的差异表达情况。
实验目的：使用VN1203致病性突变体NS1截短124株，获取可用于三次重复转录组分析的样本。
实验概述：
· 采样时间点：感染后0、3、7、12、18及24小时（hours post infection, hpi）。
· 所有实验均设置三次生物学重复。
· 三次重复定义为：使用同一批次细胞悬液铺板，于同一时间接种的3个独立培养孔。
· 与感染组时间匹配的模拟感染样本，亦采用与其余实验组相同批次的细胞悬液设置三次重复。
· 模拟感染所用的培养基与病毒原液的溶剂保持一致。

Calu-3细胞分别感染NS1截短124株(NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1))或进行模拟感染，分别于感染后0、3、7、12、18及24小时采集样本。另有一组Calu-3细胞感染野生型(Wild Type, WT: A/Vietnam/1203/2004 (H5N1))，仅于感染后7及24小时采集样本。每个时间点均设置3份模拟感染样本（本数据集中包含其中2份）与3份感染组重复样本。本研究完成了全转录组表达谱的检测分析。