Genome‑wide analysis of sex‑specifc diferences in the mother–child PELAGIE cohort exposed to organophosphate metabolites

In this project exposuere to DiethylPhosphate (DE) metabolites has been monitored in the usine of pregnant women of the French mother-child cohort PELAGIE. We performed ChIPs against H3K4me3 from male or female newborns' placenta in order to identify whether exposure to DE can affect their epigenetic landscape. Representing samples from each group were selected for genome-wide ChIP-seq analysis. Exposure and epigenetic data are compared and associated with potential effects on developmental processes. We provided 18 libraries (17 ChIPs + 1 input) for single-end sequencing (1x50bp). ChIP was performed from chromatin obtained from placenta of human male new borns. We provided 14 libraries (14 ChIPs) for single-end sequencing (1x50bp). ChIP was performed from chromatin obtained from placenta of human female new borns.

本项目在法国PELAGIE母胎队列的孕妇群体中，对二乙基磷酸（DiethylPhosphate，DE）代谢物的暴露情况进行了监测。为探究DE暴露是否会影响胎儿的表观遗传图谱，本研究针对男女新生儿的胎盘样本开展了抗组蛋白H3第4位赖氨酸三甲基化（H3K4me3）的染色质免疫沉淀（ChIP）实验。每组选取具有代表性的样本进行全基因组染色质免疫沉淀测序（ChIP-seq）分析。随后对暴露数据与表观遗传数据进行比对，并分析二者与发育过程潜在效应的关联。本研究构建了18个测序文库（17个ChIP文库+1个Input对照文库），用于单端测序（1×50bp），这些实验的染色质均取自人类男性新生儿的胎盘组织。此外还构建了14个ChIP测序文库，用于单端测序（1×50bp），其染色质取自人类女性新生儿的胎盘组织。