Dataset - chemokines, cytokines, and biomarkers in the saliva of children with Sjögren’s syndrome

INTRODUCTION
Sjögren’s syndrome can occur in children. While the concentrations of chemokines, cytokines, and biomarkers (CCBMs) are reported in the saliva of adults with Sjögren’s syndrome, this data is not known in the saliva of children with Sjögren’s syndrome. We hypothesized that CCBMs associated with lymphocyte and mononuclear cell functions were present in the saliva of children with Sjögren’s syndrome.

This dataset is associated with 2 studies recently published by Gomez Hernandez et al. A distinguishing profile of chemokines, cytokines, and biomarkers in the saliva of children with Sjögren’s syndrome. Rheumatology (accepted January 17, 2021) and Hikkaduwa Withanage et al. Dataset - chemokines, cytokines, and biomarkers in the saliva of children with Sjögren’s syndrome. Data in Brief (submitted January 17, 2021).

METHODS
Saliva was collected from 11 children diagnosed with Sjögren’s syndrome and 16 normal healthy children. The presence and concentrations of 105 CCBMs (pg/ml) were determined using multiplex fluorescent microparticle-based immunoassays.

CCBM concentrations were interpolated from their MFI values using five parameter logistic curves created from the standard concentrations and their respective MFI readings using xPonent v3.1 software (Luminex, Austin, TX) or using Milliplex Analyst v5.1 software (EMD Millipore, Billerica, MA). Occasionally, there were CCBM concentrations below the standard curve. These concentrations were extrapolated from their MFI values using curves created from zero concentration to the lowest standard concentration and their respective MFI readings. For example, CCL27 concentrations in saliva were below the standard curve. After extrapolation, the mean (standard error of the mean), minimum - maximum values were 4.6 (0.8), 1.7-9.7 pg/ml for children with Sjögrens syndrome and 2.6 (0.3), 0.6-5.8 pg/ml for normal healthy children.
There were also CCBM concentrations above the standard curve. These concentrations were extrapolated from their MFI values using curves extended beyond the highest concentration and their respective MFI readings. For example, B2M concentrations in saliva were above the standard curve. After extrapolation, the mean (standard error of the mean), minimum - maximum values were 122,671.6 (14,485.0), 98,850.0-266,527.2 pg/ml for children with Sjögrens syndrome and 98,060.6 (6,038.4), 9,110.0-111,270.0 pg/ml for normal healthy children.

RESULTS
43/105 of CCBMs were significantly different between these 2 groups and 71/105 CCBMs were not. The 43 CCBMs were associated with leukocyte chemotaxis, migration, proliferation, and regulation of T-cell activation.

CONCLUSION
This dataset is among the first to report the concentrations of CCMBs in the saliva of children with Sjögren’s syndrome. It will assist others in their work to understand the etiopathogenesis of Sjögren’s syndrome in children, and assist in the identification of early markers of disease in children.

引言

干燥综合征（Sjögren’s syndrome）可发生于儿童群体。目前已有关于成人干燥综合征患者唾液中趋化因子、细胞因子及生物标志物（CCBMs）浓度的相关报道，但儿童干燥综合征患者唾液中的此类标志物数据仍属空白。本研究推测，与淋巴细胞及单核细胞功能相关的CCBMs可在儿童干燥综合征患者的唾液中被检测到。

本数据集关联两项近期发表的研究：Gomez Hernandez等人于2021年1月17日被接收发表于《风湿病学（Rheumatology）》的《儿童干燥综合征患者唾液中趋化因子、细胞因子及生物标志物的特征谱》，以及Hikkaduwa Withanage等人于2021年1月17日提交至《数据简报（Data in Brief）》的《儿童干燥综合征患者唾液中趋化因子、细胞因子及生物标志物数据集》。

研究方法

本研究采集了11名确诊干燥综合征患儿及16名健康对照儿童的唾液样本。采用多重荧光微球免疫分析法，对105种CCBMs的浓度（单位：pg/ml）进行检测。

CCBMs的浓度通过标准浓度及其对应的平均荧光强度（MFI）值构建的五参数逻辑斯蒂曲线，借助xPonent v3.1软件（Luminex公司，美国德克萨斯州奥斯汀市）或Milliplex Analyst v5.1软件（EMD Millipore公司，美国马萨诸塞州比勒里卡市），由MFI值插值计算得到。部分CCBMs的浓度低于标准曲线下限，此时采用以零浓度至最低标准浓度及其对应MFI值构建的曲线进行外推计算。例如，唾液中CC趋化因子配体27（CCL27）的浓度低于标准曲线下限，经外推后，干燥综合征患儿组的浓度均值（标准误）、最小值-最大值范围分别为4.6（0.8）、1.7~9.7 pg/ml，健康对照组则为2.6（0.3）、0.6~5.8 pg/ml。

另有部分CCBMs的浓度高于标准曲线上限，此时采用延伸至高浓度标准之外的曲线及其对应MFI值进行外推计算。例如，唾液中β2微球蛋白（B2M）的浓度高于标准曲线上限，经外推后，干燥综合征患儿组的浓度均值（标准误）、最小值-最大值范围分别为122671.6（14485.0）、98850.0~266527.2 pg/ml，健康对照组则为98060.6（6038.4）、9110.0~111270.0 pg/ml。

研究结果

105种CCBMs中，共有43种在两组间存在显著差异，剩余71种无显著差异。这43种差异表达的CCBMs与白细胞趋化、迁移、增殖及T细胞活化调控密切相关。

研究结论

本数据集是首批报道儿童干燥综合征患者唾液中CCBMs浓度的数据集之一，可为阐明儿童干燥综合征的发病机制提供研究支撑，并助力儿童干燥综合征早期疾病标志物的筛选与鉴定。