RNA-seq data from the trachea epithelial cells of two inbred chicken lines at three time points after challenge with lentogenic Newcastle disease virus

This study aimed to characterize differential responses between the relatively resistant (Fayoumi) and susceptible (Leghorn) chicken lines when challenged with a high titered lentogenic Newcastle Disease Virus (NDV) strain via the eyes and nostrils at three weeks of age. The trachea epithelial cells were collected from challenged and non-challenged birds from each line at 2, 6, and 10 days-post-infection (dpi). High quality RNA was isolated, used for cDNA library construction, and sequenced on the HiSeq2500.

本研究旨在表征相对抗病的法尤姆鸡（Fayoumi）与易感的来亨鸡（Leghorn）品系在3周龄时经眼鼻途径攻以高滴度低毒力新城疫病毒（Newcastle Disease Virus, NDV）毒株后的差异应答反应。分别于感染后2、6、10天（days-post-infection, dpi），从两个品系的攻毒组与非攻毒组鸡只体内采集气管上皮细胞。提取高质量RNA后用于构建cDNA文库，并在HiSeq2500平台上完成测序。