Imaging Ng2:DsRed+ Murine Retina over 6 Hours in Culture

Murine retinal explant from an Ng2/Cspg4:DsRed/+ animal cultured in Dulbecco's Modified Eagle Medium (DMEM) + 5% Fetal Bovine Serum (FBS) + 1% penicillin-streptomycin at 37 degrees Celsius for 6 hours. Explants were cultured on a glass-bottom petri dish and imaged every hour on a Zeiss LSM 880 point-scanning confocal microscope. Volumetric images taken through the z-axis were acquired at 40x and 63x. Images in the figure are compressed z-stacks within a selected range, but the raw image files in .czi format are also included.

取自Ng2/Cspg4:DsRed/+动物（Ng2/Cspg4:DsRed/+ animal）的视网膜外植体（murine retinal explant），于37℃条件下在添加5%胎牛血清（Fetal Bovine Serum，FBS）及1%青霉素-链霉素（penicillin-streptomycin）的杜尔贝科改良伊格尔培养基（Dulbecco's Modified Eagle Medium，DMEM）中培养6小时。外植体培养于玻璃底培养皿（glass-bottom petri dish），并通过蔡司LSM 880点扫描共聚焦显微镜（Zeiss LSM 880 point-scanning confocal microscope）每小时成像一次。沿z轴（z-axis）采集的体积图像（volumetric images）分别在40倍和63倍放大倍数下获取。图中图像为选定范围内的压缩z-stack（compressed z-stack），但亦包含.czi格式的原始图像文件。