cDC1 derived IL27 regulates the transcriptional profile of SI-LP Th subsets

To assess the broader impact of cDC1 derived IL-27 on the properties of SI-LP CD4+ T cells, these cells were sorted from Xcr1-cre.p28fl/fl mice and p28fl/fl controls (4 mice/group) and subjected to single cell RNA sequencing (scRNA-seq). Single cell RNA sequencing of CD4 T cells isolated from mouse small-instestine lamina propria, in p28fl/fl controls (wild-type) and Xcr1-cre.p28fl/f mice

为评估经典树突状细胞1（cDC1）来源的白细胞介素27（IL-27）对小肠固有层（SI-LP）CD4阳性T细胞特性的广泛影响，研究人员从Xcr1-cre;p28fl/fl基因工程小鼠及p28fl/fl野生型对照小鼠（每组4只）中分选得到此类细胞，并进行单细胞RNA测序（scRNA-seq）。本研究同时对取自p28fl/fl野生型对照小鼠与Xcr1-cre;p28fl/fl小鼠小肠固有层的分离CD4阳性T细胞实施了单细胞RNA测序。