Table_3_Dual RNA Sequencing Reveals the Genome-Wide Expression Profiles During the Compatible and Incompatible Interactions Between Solanum tuberosum and Phytophthora infestans.xlsx

Late blight, caused by Phytophthora infestans (P. infestans), is a devastating plant disease. P. infestans genome encodes hundreds of effectors, complicating the interaction between the pathogen and its host and making it difficult to understand the interaction mechanisms. In this study, the late blight-resistant potato cultivar Ziyun No.1 and the susceptible potato cultivar Favorita were infected with P. infestans isolate SCPZ16-3-1 to investigate the global expression profiles during the compatible and incompatible interactions using dual RNA sequencing (RNA-seq). Most of the expressed Arg-X-Leu-Arg (RXLR) effector genes were suppressed during the first 24 h of infection, but upregulated after 24 h. Moreover, P. infestans induced more specifically expressed genes (SEGs), including RXLR effectors and cell wall-degrading enzymes (CWDEs)-encoding genes, in the compatible interaction. The resistant potato activated a set of biotic stimulus responses and phenylpropanoid biosynthesis SEGs, including kirola-like protein, nucleotide-binding site-leucine-rich repeat (NBS-LRR), disease resistance, and kinase genes. Conversely, the susceptible potato cultivar upregulated more kinase, pathogenesis-related genes than the resistant cultivar. This study is the first study to characterize the compatible and incompatible interactions between P. infestans and different potato cultivars and provides the genome-wide expression profiles for RXLR effector, CWDEs, NBS-LRR protein, and kinase-encoding genes.

由致病疫霉（Phytophthora infestans，P. infestans）引发的晚疫病是一种毁灭性植物病害。致病疫霉基因组编码数百个效应子，使得病原菌与寄主的互作过程趋于复杂，相关互作机制难以解析。本研究选取抗晚疫病马铃薯品种紫云1号（Ziyun No.1）与感病品种费乌瑞它（Favorita），采用致病疫霉菌株SCPZ16-3-1进行接种，通过双转录组测序（dual RNA sequencing, RNA-seq）分析亲和与非亲和互作过程中的全基因组表达谱。研究发现，多数表达的Arg-X-Leu-Arg（RXLR）效应子基因在侵染初始24小时内被抑制，24小时后则呈现上调表达。此外，在亲和互作中，致病疫霉诱导产生更多特异性表达基因（SEGs），包括RXLR效应子与细胞壁降解酶（cell wall-degrading enzymes, CWDEs）编码基因。抗病马铃薯品种激活了一系列生物胁迫响应及苯丙烷类生物合成相关特异性表达基因，涵盖kirola样蛋白、核苷酸结合位点-亮氨酸富集重复（nucleotide-binding site-leucine-rich repeat, NBS-LRR）蛋白、抗病相关及激酶编码基因。与之相反，感病马铃薯品种较抗病品种上调了更多激酶与病程相关基因。本研究首次解析了致病疫霉与不同马铃薯品种间的亲和与非亲和互作，并为RXLR效应子、CWDEs、NBS-LRR蛋白及激酶编码基因提供了全基因组表达谱数据。