Real-time quantitative PCR analysis of expression profiles of dual-specificity phosphatase family members in mouse tissues. Mus musculus

Mouse tissues (including heart, skeletal muscle, brain, lung, stomach, small instestine, colon, liver, kidney, testis, uterus, bone marrow, thymus, lymph node, spleen and embryo) were isolated from three C57BL/6 mice (eight-week-old, uterus from female and other tissues from male). We used Applied Biosystems 7500/7500 Fast Real-Time PCR Systems to quantitate expression levels of DUSP family members in the indicated mouse tissues. Overall design: qPCR gene expression profiling. Various tissues pooled from three C57BL/6 mice were used as indicated in the summary. Equal amount total RNA from each tissue was pooled prior to gene expression analysis.

从3只8周龄C57BL/6小鼠中分离得到15种小鼠组织，包括心脏、骨骼肌、脑、肺、胃、小肠、结肠、肝脏、肾脏、睾丸、子宫、骨髓、胸腺、淋巴结、脾脏及胚胎；其中子宫组织取自雌性小鼠，其余组织均取自雄性小鼠。采用应用生物系统（Applied Biosystems）7500/7500 Fast实时荧光定量PCR系统，对指定小鼠组织中双特异性磷酸酶（DUSP）家族成员的表达水平进行定量检测。实验整体设计：定量聚合酶链反应（qPCR）基因表达谱分析。按摘要所示，使用3只C57BL/6小鼠的多种混合组织开展实验；在开展基因表达分析前，将各组织的总RNA按等量比例混合后再进行检测。