Monosaccharide transporter OsMST6 is activated by transcription factor OsERF120 to enhance chilling tolerance in rice seedlings

Chilling stress caused by extreme weather is threatening global rice (Oryza sativa L.) production. Identifying components of the signal transduction pathways underlying chilling tolerance in rice would advance molecular breeding. Here, we report that OsMST6, which encodes a monosaccharide transporter, positively regulates the chilling tolerance of rice seedlings. The mst6 mutants showed hypersensitivity to chilling, while the OsMST6 overexpression lines were tolerant. During chilling stress, OsMST6 transported more glucose into cells to modulate sugar and ABA signal pathways. We showed that the transcription factor OsERF120 could bind to the DRE/CRT element of the OsMST6 promoter and activate the expression of OsMST6 to positively regulate chilling tolerance. Genetically, OsERF120 was functionally dependent on OsMST6 when promoting chilling tolerance. In summary, OsERF120 and OsMST6 form a new downstream chilling regulatory pathway in rice in response to chilling stress, providing valua..., The 14-day-old rice seedlings of ZH11 and mst6-1, incubated in the greenhouse with 28 °C/25 °C (10 h day/14 h night) cycles in Kimura B nutrient solution, were treated for 0 h and 4 h at 4 °C, and the whole seedlings were sampled for transcriptome sequencing with three biological replicates. 
RNA-seq was performed at Anoroad Genome (Beijing, China). Clean reads were mapped referring to the rice genome (Oryza sativa IRGSP-1.0.38), using HISAT2 (Sirén et al., 2014) with default parameters. The significant differentially expressed genes were determined using DESeq2 with |Fold change| ≥2, adjusted P value <0.05. Gene ontology (GO) analysis was performed using DAVID., , # Monosaccharide transporter *OsMST6* is activated by transcription factor OsERF120 to enhance chilling tolerance in rice seedlings

[https://doi.org/10.5061/dryad.280gb5mw1](https://doi.org/10.5061/dryad.280gb5mw1)

We have submitted our data, which are the results of transcriptome data analysis for *mst6-1* (a mutant of the monosaccharide transporter *OsMST6*) and wild-type ZH11 rice seedlings. The 14-day-old rice seedlings of *mst6-1* and ZH11 were chill-treated for 0 h and 4 h at 4 °C, then the entire seedlings were sampled for transcriptome sequencing and analysis with three biological replicates.

The data include the differentially expressed genes (**Dataset_1_DEGs_in_ZH11_and_mst6-1.xlsx**), the significantly enriched terms of KEGG (Kyoto Encyclopedia of Genes and Genomes) and GO (Gene Ontology) analysis results (**Dataset_2_KEGG_and_GO_terms.xlsx**), and enriched genes from the ABA-activated signaling pathway and PP2C family (**Dataset_3_Gene_information_of_ABA-activated_signal...

极端天气引发的低温胁迫正威胁全球水稻（Oryza sativa L.）的生产。解析水稻低温耐受相关信号转导通路的组分，将推动分子育种研究进展。本研究发现，编码单糖转运蛋白的OsMST6可正向调控水稻幼苗的低温耐受性：mst6突变体对低温表现出超敏反应，而过表达OsMST6的株系则具备低温耐受能力。在低温胁迫过程中，OsMST6可将更多葡萄糖转运至细胞内，以调控糖信号与脱落酸（ABA）信号通路。本研究证实，转录因子OsERF120能够结合OsMST6启动子的DRE/CRT元件，激活OsMST6的表达，进而正向调控水稻低温耐受性。从遗传层面而言，OsERF120在提升水稻低温耐受性的过程中，其功能依赖于OsMST6。综上，OsERF120与OsMST6在水稻中构建了一条全新的下游低温调控通路，以响应低温胁迫，为...

将在温室中以28℃/25℃（昼10小时/夜14小时）周期培养、于木村B营养液中生长14天的ZH11与mst6-1水稻幼苗，置于4℃环境下分别处理0小时与4小时，采集全株幼苗样本进行转录组测序（RNA-seq），设置3次生物学重复。

转录组测序由北京诺禾致源基因组学（Anoroad Genome，中国北京）完成。参照水稻参考基因组（Oryza sativa IRGSP-1.0.38），使用默认参数的HISAT2（Sirén et al., 2014）对清洁读段（clean reads）进行比对分析。利用DESeq2筛选显著差异表达基因，筛选标准为|折叠变化|≥2且校正后P值<0.05。采用DAVID进行基因本体（GO）富集分析。

# 单糖转运蛋白OsMST6被转录因子OsERF120激活，增强水稻幼苗低温耐受性

https://doi.org/10.5061/dryad.280gb5mw1

本研究已提交相关数据，即单糖转运蛋白OsMST6的突变体mst6-1与野生型ZH11水稻幼苗的转录组数据分析结果。将mst6-1与ZH11的14日龄水稻幼苗置于4℃低温环境下分别处理0小时与4小时后，采集全株幼苗样本进行转录组测序与分析，设置3次生物学重复。

本数据集包含：差异表达基因文件（**Dataset_1_DEGs_in_ZH11_and_mst6-1.xlsx**）、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）及基因本体（Gene Ontology, GO）富集分析的显著富集条目文件（**Dataset_2_KEGG_and_GO_terms.xlsx**），以及脱落酸（ABA）激活信号通路与PP2C家族相关富集基因文件（**Dataset_3_Gene_information_of_ABA-activated_signal...**）