Blood loss leads to increase in relative abundance of opportunistic pathogens in the gut microbiome of rabbits. Gut microbiome

We used NewZealand rabbits to create a blood loss complication and designed a five-time-point fecal sampling strategy including 24-hour pre-blood loss treatment, 24-hour, 36-hour, 48-hour and 1-week post-blood loss treatment. Gut microbiome composition and diversity were analyzed using 16S rRNA V1-V2 gene sequencing and downstream α-diversity, β-diversity, and taxonomy analysis. This study aims to determine whether acute blood loss affects the gut microbiome and the dynamic variation of the gut microbiome following the loss of blood.

本研究以新西兰兔（New Zealand rabbit）为实验动物，构建失血性并发症模型，并设计了五个时间点的粪便采样方案，涵盖失血前24小时以及失血后24小时、36小时、48小时与1周共五个采样节点。采用16S rRNA V1-V2区基因测序（16S rRNA V1-V2 gene sequencing）技术，结合下游α多样性、β多样性及分类学分析，对肠道菌群的组成与多样性进行解析。本研究旨在明确急性失血是否会对肠道菌群产生影响，以及失血后肠道菌群的动态变化规律。