Hellbender skin prokaryote transcriptome

In this study, we explored the efficacy of Bd vaccination on hellbender salamanders and functionally characterized host, host-associated microbiome, and pathogen throughout immunization and infection. We compared the function of hellbender skin, the skin microbiome, and Bd with a metatranscriptome approach at four experimental time points, (i) pre-vaccination and pre-infection, (ii) post-vaccination and pre-infection, (iii) early infection, and (iv) late infection.

本研究探究了Bd疫苗接种对隐鳃鲵蝾螈的有效性，并在免疫与感染全过程中对宿主、宿主相关微生物组及病原体进行了功能表征。我们采用宏转录组方法，在四个实验时间点比较了隐鳃鲵皮肤、皮肤微生物组及Bd的功能：（i）疫苗接种前与感染前，（ii）疫苗接种后与感染前，（iii）早期感染，（iv）晚期感染。