DataSheet_1_Metabolic profiling and expression analysis of key genetic factors in the biosynthetic pathways of antioxidant metabolites in mungbean sprouts.docx

Mungbeans (Vigna radiata L.), a major legume crop in Asia, contain higher amounts of functional substances than other legumes, such as catechin, chlorogenic acid, and vitexin. Germination can improve the nutritional value of legume seeds. Here, 20 functional substances were profiled in germinated mungbeans and the expression levels of the transcripts of key enzymes in targeted secondary metabolite biosynthetic pathways were identified. VC1973A, a reference mungbean elite cultivar, had the highest amount of gallic acid (99.93 ± 0.13 mg/100 g DW) but showed lower contents of most metabolites than the other genotypes. Wild mungbeans contained a large amount of isoflavones compared with cultivated genotypes, especially for daidzin, genistin and glycitin. The expression of key genes involved in biosynthetic pathways had significant positive or negative correlations with the target secondary metabolite contents. The results indicate that functional substance contents are regulated at the transcriptional level, which can be applied to improve the nutritional value of mungbean sprouts in molecular breeding or genetic engineering, and wild mungbeans are a useful resource to improve the quality of mungbean sprouts.

绿豆（Vigna radiata L.）作为亚洲主要豆科作物，其功能性物质含量高于其他豆科物种，例如儿茶素、绿原酸与牡荆素。发芽处理可提升豆科种子的营养价值。本研究对发芽绿豆中的20种功能性物质开展了靶向表征，并对目标次生代谢物生物合成通路中关键酶的转录本表达水平进行了检测。参比优良绿豆品种VC1973A的没食子酸含量最高，达99.93 ± 0.13 mg/100 g 干重（DW），但多数代谢物的含量低于其他供试基因型。与栽培基因型相比，野生绿豆的异黄酮类物质含量更为丰富，尤其是大豆苷、染料木苷与黄豆黄苷。生物合成通路中的关键基因表达水平与目标次生代谢物含量存在显著的正相关或负相关关系。本研究结果表明，功能性物质的含量在转录层面受到调控，该发现可应用于通过分子育种或基因工程手段提升绿豆芽的营养价值；同时，野生绿豆是改良绿豆芽品质的优质种质资源。