Biochemical analysis of H2O2-induced mutation spectra revealed that multiple damages were involved in the mutational process

This dataset is associated with the paper entitled "<i>Biochemical analysis of H2O2-induced mutation spectra revealed that multiple damages were involved in the mutational process</i>", by Sugiyama and Sanyal."Fastq.zip" contains all fastaq raw data files that have been used in the paper. Fastaq file name contains NGS run name and barcode # (e.g. 22A_BC021.fastq). These identifications are used in Table S1 of the paper, which summarizes experimental conditions."NGS Protocol 4.4.docx" is our laboratory protocol for basic analysis of our NGS data. This protocol is designed for new students to learn how to obtain mutation frequency from fastaq data. The protocol uses Excel template files with macro, which are also included in this dataset. The protocol and macros are designed for the specific data analysis, not for general use. Macro works only for specific template sequences used in the paper.

本数据集关联Sugiyama与Sanyal发表的题为《过氧化氢诱导突变谱的生化分析揭示突变过程涉及多种损伤》的论文。压缩包Fastq.zip内含本论文所用的全部FASTQ原始数据文件。FASTQ文件名包含下一代测序（Next-Generation Sequencing，NGS）运行标识与条形码编号（例如22A_BC021.fastq）。此类标识已在论文的表S1中使用，该表汇总了所有实验条件。文档《NGS Protocol 4.4.docx》为本实验室用于NGS数据基础分析的实验规程，专为新生设计，用于学习如何从FASTQ数据中提取突变频率。该规程配套带宏的Excel模板文件，此类文件也包含在本数据集内。本规程与宏程序均为针对本次特定数据分析所开发，不具备通用适用性，且宏程序仅可用于本论文中使用的特定模板序列。