Characteristics of tuberculosis patients (n = 547) by presence of Euro-American lineage or MIRU-VNTRplus spoligotype clonal complex (CC and Singletons) M. tuberculosis strains.

1 The MIRU-VNTR plus web application (http://www.miru-vntrplus.org) was used to analyse spoligotype data of the M. tuberculosis isolates [39–40]. For minimum spanning tree analysis the the Kruskal’s algorithm and force-directed graph layout for visualization according to the SIT using one single locus variation from the Node, SLV(1) was used to generate groupings into clonal clusters (CC) and Singletons (non-grouped) (Table 1).2 Statistical models: generalized linear model, Tuberculosis with a Euro-American lineage strain ~ HIV infection + MDRTB + gender + age group, and Tuberculosis with strains from MIRU-VNTR-plus spoligotype derived clonal complexes ~ HIV infection + MDRTB + gender + age groupNote: Significance codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ‘ 1MDRTB = multidrug resistant tuberculosisCharacteristics of tuberculosis patients (n = 547) by presence of Euro-American lineage or MIRU-VNTRplus spoligotype clonal complex (CC and Singletons) M. tuberculosis strains.

1 本研究采用MIRU-VNTR plus网络应用程序（http://www.miru-vntrplus.org）分析结核分枝杆菌（M. tuberculosis）分离株的间隔寡核苷酸分型（spoligotype）数据[39-40]。在最小生成树分析中，采用克鲁斯卡尔算法（Kruskal’s algorithm），依据共享型别（Spoligotype International Type, SIT），以节点单基因座变异（Single Locus Variation, SLV(1)）作为指标进行可视化的力导向图布局，将菌株划分为克隆簇（Clonal Cluster, CC）与单例株（非分组菌株）两类（表1）。
2 统计模型：构建两类广义线性模型（Generalized Linear Model），其一为「携带欧美系谱系（Euro-American lineage）菌株的结核病～HIV感染+耐多药结核病（MDRTB）+性别+年龄组」，其二为「携带MIRU-VNTR plus间隔寡核苷酸分型衍生克隆复合体菌株的结核病～HIV感染+耐多药结核病+性别+年龄组」。
注：显著性代码规则：0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1；其中MDRTB为耐多药结核病（multidrug resistant tuberculosis）的缩写。
本研究按感染菌株为欧美系谱系结核分枝杆菌，或属于MIRU-VNTR plus间隔寡核苷酸分型衍生克隆簇（CC）与单例株的情况，对547名结核病患者的临床特征进行统计分析。