Genome-wide transcriptional analysis of the primitive erythroid (EryP) lineage differentiation

During mammalian postimplantation development, primitive erythroblasts (EryP) or red blood cells are the first cell type to be specified from nascent mesoderm, late in gastrulation. EryP arise in the yolk sac from a transient wave of progenitors, enter the circulation soon thereafter, and continue to mature in a stepwise and synchronous fashion. We have taken advantage of a transgenic mouse line in which a green fluorescent reporter is expressed specifically in the EryP lineage to identify and purify EryP at different stages of maturation from E7.5 through E12.5. A transcriptional profile that encompasses key developmental landmarks of this lineage was determined using a genome-wide microarray analysis (Illumina platform). Dataset is a lineage-specific transcription profiling of EryP development on consecutive days from E7.5 through E12.5. At least 3 independent biological replicates were used for each developmental time point.

在哺乳动物植入后发育过程中，原始红细胞（primitive erythroblasts，简称EryP）即红细胞系，是原肠胚形成晚期最先由新生中胚层分化特化的细胞类型。原始红细胞由一过性造血祖细胞群在卵黄囊中产生，随后迅速进入血液循环，并以逐步且同步的方式持续成熟。本研究利用一株绿色荧光报告基因特异性在EryP谱系中表达的转基因小鼠品系，对胚胎发育第7.5天（E7.5）至第12.5天（E12.5）期间不同成熟阶段的原始红细胞进行鉴定与纯化。本研究通过全基因组微阵列分析（Illumina平台），获取了涵盖该谱系关键发育节点的转录组特征。本数据集为E7.5至E12.5期间连续天数的EryP发育谱系特异性转录谱。每个发育时间点均设置至少3次独立生物学重复。