Stimulation with M.tb peptide pool induces multiple signaling pathways involved in providing protection during M.tb infection

M.tb Peptide pool induces an intricate network of signaling pathways in DCs and T cells which leads to enhanced cytokine production and T cell activation. To understand the host changes at the transcription levels leading to an increased T cell activation and an augmented pro-inflammatory cytokine upon M.tb peptide pool treatment, we performed comparative transcriptome analysis of unstimulated dendritic cells DCs (Un-DC) vs peptide pool pulsed DCs (Pep-DC) and T cells co-cultured with unstimulated DCs (Un-TC) vs T cells co-cultured with peptide pool pulsed DCs (Pep-TC).

结核分枝杆菌（Mycobacterium tuberculosis，M.tb）肽池可在树突状细胞（dendritic cells, DCs）与T细胞中诱导出复杂的信号通路网络，进而促进细胞因子产生并增强T细胞活化。为阐明经M.tb肽池处理后，宿主在转录水平上发生的、可增强T细胞活化与提升促炎细胞因子水平的分子变化，我们对未受刺激的树突状细胞（Un-DC）与经肽池脉冲致敏的树突状细胞（Pep-DC），以及与未受刺激树突状细胞共培养的T细胞（Un-TC）、与经肽池脉冲致敏树突状细胞共培养的T细胞（Pep-TC）开展了比较转录组学分析。