Composition and variation of respiratory microbiota in healthy military personnel Metagenome

Certain occupational and geographical exposures have been associated with an increased risk of lung disease. As a baseline for future studies, we sought to characterize the upper respiratory microbiome of healthy military personnel in a garrison environment. Nasal, oropharyngeal and nasopharyngeal swabs were collected from 50 healthy active duty volunteers eight times over the course of one year and subjected to pyrosequencing of 16S rDNA V3 to V1 region. The study produced a large set of pyrosequencing data of bacterial 16S rDNA V3-V1 region for respiratory microbiome of healthy active duty service members. Pre-processing of sequencing reads shows good data quality. The derived microbiome profiles are consistent both internally and with previous reports, suggesting utility for further analyses and association studies using sequence and demographic data.

多种职业暴露与地理环境暴露均被证实与肺部疾病风险升高相关。为给后续研究提供基准数据集，本研究旨在刻画卫戍环境下健康军人的上呼吸道微生物组特征。研究团队于一年内从50名健康现役志愿者中分8次采集鼻腔、口咽及鼻咽拭子样本，并对细菌16S rDNA V3至V1区进行焦磷酸测序（Pyrosequencing）。本研究生成了针对健康现役军人呼吸道微生物组的大规模细菌16S rDNA V3-V1区焦磷酸测序数据集。测序读段的预处理结果显示数据质量优异。所得微生物组谱不仅内部一致性良好，且与既往研究结果相符，表明该数据集可用于后续结合测序数据与人口统计学数据的分析及关联研究。