The dural innate immune barrier protects the CNS from systemic virus invasion [MHCII_10X]

To investigate heterogeneity of MHCII high CNS border-associated mononuclear phagocytes (BAMPs), MHCII high BAMPs from 30 adult male mice were FACS sorted and single-cell RNA seqs were performed MHCII high CNS BAMPs from 30 adult male mice were sorted and single-cell libraries were made

为探究MHCII高表达的中枢神经系统（Central Nervous System, CNS）边界相关单核吞噬细胞（BAMPs）的异质性，研究人员从30只成年雄性小鼠中分离得到MHCII高表达的BAMPs，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）完成分选纯化，随后开展单细胞RNA测序并构建单细胞文库。