Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes. Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

Transcriptional profiling of undifferentiated MSCs, MSCs in 3D culture, early differentiation time-points 15m, 30m, 1h, 2h, 4h, 8h, 16h after induction with TGF-β3 and hyaluronic acid, and fully differentiated chondrocytes (21 days after induction) using RNA-seq in triplicates of clones.

本数据集通过RNA测序（RNA-sequencing, RNA-seq）完成转录组分析，每个克隆设置三次生物学重复，所分析的样本包括：未分化间充质干细胞（Mesenchymal Stem Cells, MSCs）、三维培养的间充质干细胞、经转化生长因子-β3（Transforming Growth Factor-β3, TGF-β3）与透明质酸（hyaluronic acid）诱导分化后的早期时间点样本（15分钟、30分钟、1小时、2小时、4小时、8小时、16小时），以及诱导分化21天后的完全分化软骨细胞。