Sequencing data for ribozyme-engineered CVS-N2cΔG rabies virus barcode libraries

This dataset contains assembled barcode sequencing reads from ribozyme-engineered CVS-N2cΔG rabies virus barcode libraries designed for monosynaptic retrograde neural circuit tracing. Dataset contents: - raw_in_vitro/ — assembled barcode sequences from packaged viral libraries prior to in vivo injection - raw_in_vivo/ — assembled barcode sequences recovered from mouse primary visual cortex (V1) and lateral geniculate nucleus (LGN) following stereotaxic injection These data support the evaluation of barcode diversity, effective capacity, collision rates, and trans-synaptic transmission fidelity across ribozyme configurations.