Fig1B_qPCR_G6PD_WT-A549_R3_LG400.xlsx

A549 or A549 Xrn1 knock-out cells were either mock infected, infected with WT influenza A/Puerto Rico/8/1934 (H1N1) virus ("PR8") or with PA-X deficient PR8-PA(∆X) for 16 hours before RNA extraction. mRNA levels of influenza A virus HA and human G6PD, a PA-X target, were quantified by qRT-PCR, normalized by 18S rRNA levels

将A549细胞与A549 Xrn1基因敲除细胞分为三组处理：分别接受模拟感染、野生型甲型流感病毒A/Puerto Rico/8/1934（H1N1，简称PR8）感染，以及PA-X缺陷型PR8-PA(∆X)感染，感染时长均为16小时，随后提取总RNA。采用qRT-PCR定量检测甲型流感病毒HA以及PA-X靶标人G6PD的mRNA水平，检测结果以18S rRNA水平进行归一化校正。