Synergistic effect and mechanism study of Phycocyanin photosensitizer combined with Selenium against lung tumor in mouse. Synergistic effect and mechanism study of Phycocyanin photosensitizer combined with Selenium against lung tumor in mouse

We used selenium as a photodynamic anti-tumor synergist of phycocyanin to explore its inhibitory effect on lung cancer and its molecular mechanism in vitro. First of all, we used LLC-luc mouse lung cancer cells to establish a tumor-bearing model. Selenium-enriched phycocyanin was injected next to the tumor. When it was absorbed by the tumor tissue, the tumor site was irradiated by a 620nm wavelength laser. The changes in tumor size were monitored in real-time and the physiological indexes of mice were measured. It was found that selenium phycocyanin photodynamic therapy could enhance the inhibitory effect of tumors and improve the level of antioxidation in tumor-bearing mice. In addition, the pathological section observation and electron microscope microstructure analysis of the tumor tissue showed that the effect of the selenium-enriched phycocyanin photodynamic treatment group was more significant. At the same time, the tumor tissue transcriptional group sequencing analysis and qRT-PCR verification analysis showed that selenium-enriched phycocyanin photodynamic treatment group could reduce the expression of Mmp13, Serpine1, Vegfa, and Ppbp genes inhibit tumor cell metastasis and proliferation, up-regulate the expression of Ccl2, Ccl3, Cxcl2 and down-regulate the expression of Ccl24 chemokine, and promote tumor local immunity. Our results show that selenium phycocyanin photodynamic therapy plays an anti-tumor effect by promoting tumor cell apoptosis, reducing inflammation, and promoting tumor immunity. Overall design: The tumor tissues of lung cancer mice treated with SePC and PC and irradiated with 620nm Laser were the treatment group, and those irradiated with 620nm Laser were the control group. Experiments were performed in duplicate.

本研究以硒作为藻蓝蛋白（phycocyanin）的光动力抗肿瘤增效剂，体外探究其对肺癌的抑制作用及其分子机制。首先，我们采用LLC-luc小鼠肺癌细胞构建荷瘤模型，于瘤旁注射富硒藻蓝蛋白（selenium-enriched phycocyanin）。待其被肿瘤组织吸收后，采用620nm波长激光照射肿瘤部位，实时监测肿瘤体积变化并检测小鼠生理指标。研究发现，富硒藻蓝蛋白光动力疗法可增强肿瘤抑制效果，并提升荷瘤小鼠的抗氧化水平。此外，对肿瘤组织的病理切片观察与电子显微镜微观结构分析显示，富硒藻蓝蛋白光动力治疗组的干预效果更为显著。同时，通过肿瘤组织转录组测序分析与实时荧光定量反转录PCR（qRT-PCR）验证分析可知，富硒藻蓝蛋白光动力治疗组可下调Mmp13、Serpine1、Vegfa及Ppbp基因的表达，从而抑制肿瘤细胞的转移与增殖；上调Ccl2、Ccl3、Cxcl2的表达，并下调Ccl24趋化因子的表达，进而促进肿瘤局部免疫。本研究结果表明，富硒藻蓝蛋白光动力疗法可通过促进肿瘤细胞凋亡、减轻炎症反应以及增强肿瘤免疫发挥抗肿瘤作用。实验整体设计：经富硒藻蓝蛋白（SePC）与普通藻蓝蛋白（PC）处理并接受620nm激光照射的肺癌荷瘤小鼠肿瘤组织为治疗组，仅接受620nm激光照射的为对照组。实验均重复两次开展。