Development of a glycan microarray based method for ABO blood typing. Homo sapiens

Purpose: This study uses a high-throughput glycan microarray to develop a novel method to assign ABO blood type. The method will then be applied to samples from patients treated with PROSTVAC to determine if blood type correlates with survival Results: Many blood group A and B antigens correlate with blood type. Blood typing is best achieved using a combination of 10 signals Conclusion: ABO blood type can be determined with greater than 97% accuracy using only 4 microliters of serum. Overall design: Sera from 220 healthy subjects was divided into a training set (n=60), and test set (n=40), and a validation set (n=120). The training set was used to evaluate various methods for assigning blood type. The best methods were then further evaluated and refined with the test set. Finally, the best blood typing method was applied to the validation set to more extensively assess accuracy.

研究目的：本研究借助高通量聚糖微阵列（glycan microarray）技术，开发一种全新的ABO血型分型方法；随后将该方法应用于接受PROSTVAC治疗的患者样本，以探究血型是否与生存率存在相关性。研究结果：多数A、B血型抗原与血型表型具有显著相关性；采用10个检测信号的组合方案可实现最优的血型分型效果。研究结论：仅需4微升血清，即可实现准确率高于97%的ABO血型分型检测。实验整体设计：招募220名健康受试者的血清样本，将其划分为训练集（training set，n=60）、测试集（test set，n=40）与验证集（validation set，n=120）。首先利用训练集对多种血型分型方法开展评估；随后通过测试集对筛选出的最优方法进行进一步优化与完善；最终将确定的最优血型分型方法应用于验证集，以更全面地评估其分型准确率。