Transcriptional responses of normal human dermal fibroblasts (NHDF) induced by Borrelia burgdorferi sensu stricto. Homo sapiens

The aim of the study was to compare the global transcriptional responses elicited in NHDF cells by three different strains of Borrelia burgdorferi ss (the agent of Lyme borreliosis), representative of different stages in the life cycle of Borrelia: one reference strain isolated from a tick (strain N40), and two invasive strains isolated from skin biopsy of erythema migrans (strain Pbre c4) and acrodermatitis chronica atrophians skin lesions (strain 1408 c1). Overall design: Three different experimental conditions have been tested: (1) unstimulated NHDF vs NHDF stimulated by Borrelia strain N40 / (2) unstimulated NHDF vs NHDF stimulated by Borrelia strain Pbre c4 / (3) unstimulated NHDF vs NHDF stimulated by Borrelia strain 1408 c1. There is 2 biological replicates for each condition. All NHDF stimulation have been performed in independent experiments.

本研究旨在比较三种不同伯氏疏螺旋体狭义亚种（Borrelia burgdorferi ss，莱姆疏螺旋体病的致病原）菌株感染正常人皮肤成纤维细胞（NHDF）后引发的全转录组应答差异，上述三种菌株分别代表伯氏疏螺旋体生活史的不同阶段：分离自蜱虫的参考菌株N40、分离自游走性红斑皮肤活检样本的侵袭性菌株Pbre c4，以及分离自慢性萎缩性肢端皮炎皮肤病灶的侵袭性菌株1408 c1。总体实验设计：共设置三种不同实验条件：(1) 未刺激的NHDF与经伯氏疏螺旋体菌株N40刺激的NHDF；(2) 未刺激的NHDF与经伯氏疏螺旋体菌株Pbre c4刺激的NHDF；(3) 未刺激的NHDF与经伯氏疏螺旋体菌株1408 c1刺激的NHDF。每种条件设置2次生物学重复，所有NHDF刺激实验均独立开展。