Environmental DNA sample results from Santa Cruz Island, California, May 2025

We used Wilderlab eDNA kits to collect eDNA samples in a creek colloquially known as Cañada del Puerto Creek (Hydrologic Unit Code 12 number 180600140201) on Santa Cruz Island, California, in May 2025. Samples were sent to Wilderlab for metabarcoding analysis using their comprehensive panel of assays to amplify DNA from a variety of taxa, including vertebrates (fish, mammals, reptiles, amphibians, and birds), crustaceans, and freshwater insects. This dataset is the metabarcoding results, which includes exact sequent variants (ESVs) and their taxonomic assignments found in each sample using each assay.

本研究于2025年5月，在加利福尼亚州圣克鲁兹岛一条俗称卡尼亚达德尔普埃尔托溪（Cañada del Puerto Creek，水文单元代码（Hydrologic Unit Code）12号180600140201）的溪流中，使用Wilderlab环境DNA（environmental DNA, eDNA）试剂盒采集eDNA样本。随后将样本送至Wilderlab，采用其综合检测组合开展宏条形码（metabarcoding）分析，以扩增鱼类、哺乳类、爬行类、两栖类、鸟类等脊椎动物、甲壳类及淡水昆虫在内的各类分类群的DNA。本数据集即为该宏条形码分析的结果，包含每个样本通过各检测试剂盒获得的准确序列变异体（exact sequence variants, ESVs）及其分类学注释信息。