BEST-CROP Variant Call Data for the barley cultivar Carbona

Variant call data for variety Carbona in "vcf" format from the University of Milan. Genomic DNA from individuals was sequenced to approximately 40x coverage using short (2*150nt) paired end reads. Reads were trimmed using FastP and mapped to the MorexV3 assembly with Bowtie2. PCR duplicate reads were marked with Samtools and variants were called using FreeBayes. Any questions about this data please contact David Horner (University of Milan) [e-mail]. This data is released under a CC BY-NC licence. In the case of reuse of this data, please acknowledge the BestCrop project (https://www.bestcrop.eu/index.html). Data is permanently hosted and available from https://germinate.hutton.ac.uk/best-crop/#/data/data-resources来自米兰大学的Carbona品种变异检测数据，格式为VCF（Variant Call Format）。采用2×150nt的短片段双端测序读段对个体基因组DNA进行测序，测序覆盖度约为40×。使用FastP对测序读段进行质量修剪，并通过Bowtie2将其比对至MorexV3参考基因组组装序列；利用Samtools标记PCR重复读段，随后通过FreeBayes完成变异位点检测。若对该数据集存在任何疑问，请联系米兰大学的David Horner（邮箱：[e-mail]）。本数据集采用CC BY-NC（知识共享署名-非商业性使用）许可协议发布。若需复用该数据集，请注明BestCrop项目（https://www.bestcrop.eu/index.html）。 本数据集永久存储于https://germinate.hutton.ac.uk/best-crop/#/data/data-resources并可公开获取。