RNA-seq of Arabidopsis thaliana seedlings growing in the presence of a bacterial synthetic community under full and low phosphate conditions [PBI]

This study evaluates the transcriptome of Arabidopsis thaliana seedlings growing in the presence of a 35-member bacterial SynCom under different phosphate availability Arabidopsis seeds (wild-type Col-0 and the mutants phr1 and phr1 phl1) were germinated on Johnson medium containing 0.5 % sucrose with 1 mM Pi (in the form of KH2PO4) for 7 days in the vertical position. Plants were then transferred to 50 µM Pi (low) or 625 µM Pi (high) media (without sucrose) alone or with the Synthetic Community at 10E5 c.f.u / mL for another 12 days before harvesting. Plants were grown in a growth chamber in a 15-h dark/9-h light regime (21°C day /18°C night). The experiment includes a total of ten biological replicates for Col-0 and phr1 and six for phr1 phl1 from five independent experiments

本研究评估了不同磷有效性条件下，与35个成员组成的细菌合成群落（SynCom）共培养的拟南芥（Arabidopsis thaliana）幼苗的转录组。实验所用拟南芥种子包括野生型Col-0及phr1、phr1 phl1突变体，将其在含0.5%蔗糖、1 mM Pi（以KH2PO4形式提供）的Johnson培养基上垂直萌发培养7天。随后将幼苗转移至不含蔗糖的50 μM Pi（低磷）或625 μM Pi（高磷）培养基中，分别单独培养或添加浓度为10^5 c.f.u./mL的合成群落，继续培养12天后收获样本。所有植株均在生长箱内培养，光周期设置为15小时黑暗/9小时光照，昼温21℃、夜温18℃。本实验依托5次独立实验完成，其中Col-0与phr1各设置10个生物学重复，phr1 phl1设置6个生物学重复。