RNA deep sequencing data of the whole transcriptomes of the aa3 mutant, aa3 mprA double mutant, and aa3 rel double mutant strains of Mycobacterium smegmatis MC2 155

To identify the effect of additional deletion of rel and mprA in the aa3 mutant strain lacking the aa3 cytochrome c oxidase of the respiratory electron transport chain of Mycobacterium smegmatis MC2 155, we constructed the aa3 rel double mutant and aa3 mprA double mutant and profiled the transcriptomes of the aa3 mutant, aa3 mprA double mutant and aa3 rel double mutant strains. Our comparative RNA sequencing analysis revealed that expression of most ribosomal proteins was induced in the aa3 rel double mutant and aa3 mprA double mutant relative to the aa3 mutant. Comparison of gene expression between the aa3 mutant, aa3 mprA double mutant, and aa3 rel double mutant strains of Mycobacterium smegmatis that are aerobically grown.

为明确在缺失呼吸电子传递链aa3型细胞色素c氧化酶（aa3 cytochrome c oxidase）的耻垢分枝杆菌MC2 155（Mycobacterium smegmatis MC2 155）aa3突变菌株中，额外敲除rel与mprA基因的生物学效应，我们构建了aa3 rel双突变株与aa3 mprA双突变株，并对aa3单突变株、aa3 mprA双突变株及aa3 rel双突变株的转录组进行了测序分析。通过比较RNA测序分析，我们发现相较于aa3单突变株，aa3 rel双突变株与aa3 mprA双突变株中绝大多数核糖体蛋白编码基因的表达均被诱导上调。本研究的基因表达比较分析均针对需氧培养的耻垢分枝杆菌MC2 155的上述三类突变菌株展开。