RNA-seq of 32D clone 3 cells transfected with CXCR2 (pEGFP-N1-CXCR2) or an empty vector (pEGFP-N1) with CXCL1/2 treatment. RNA-seq of 32D clone 3 cells transfected with CXCR2 (pEGFP-N1-CXCR2) or an empty vector (pEGFP-N1) with CXCL1/2 treatment

Purpose: To elucidate a potential role of non-coding RNA in myeloid progenitor cells during the differentiation induced by CXCR2 activation, the 32D clone 3 cells transfected with CXCR2 to simulate GMPs and were examined by RNA sequencing Overall design: To research the function of CXCR2 in the differentiation of myeloid progenitor cells to monocytic myeloid-derived suppressor cells, we used 32D clone 3 cells transfected with CXCR2 to simulate myeloid progenitor cells. Then the cells were incubated with CXCL1 and CXCL2 for 4 h and examined by RNA sequencing. The group that 32D clone 3 cells transfected with an empty vector (pEGFP-N1) was used as the Control group, while the Treated group was transfected with CXCR2 (pEGFP-N1-CXCR2).

数据集用途：阐明非编码RNA在CXC趋化因子受体2（CXCR2）激活诱导的髓系祖细胞分化过程中的潜在作用。本研究采用转染CXCR2的32D克隆3细胞模拟粒细胞-单核细胞系祖细胞（granulocyte-monocyte progenitors, GMPs），并通过RNA测序（RNA sequencing）进行检测。
整体实验设计：为探究CXCR2在髓系祖细胞向单核细胞系髓源性抑制细胞分化过程中的功能，本研究使用转染CXCR2的32D克隆3细胞模拟髓系祖细胞。随后将细胞与CXCL1及CXCL2共孵育4小时，采用RNA测序进行检测。其中，转染空载体（pEGFP-N1）的32D克隆3细胞作为对照组，转染CXCR2重组载体（pEGFP-N1-CXCR2）的细胞为处理组。