High resolution definition of the Staphylococcus aureus WalKR regulon [RNA-seq]

Here, we use a panel of defined walKR mutants and functional genomics including ChIP-seq to confirm a high-resolution consensus WalR DNA-binding motif and characterise a 61-gene direct regulon Overall design: FLAG-tagged bacterial DNA-binding proteins under inducible expression in Staphylococcus aureus, to faciltate immunoprecipitation DNA-sequencing (ChIP-seq)

本研究采用一组经明确鉴定的walKR突变体，结合包括染色质免疫沉淀测序（ChIP-seq，Chromatin Immunoprecipitation Sequencing）在内的功能基因组学技术，确认了高分辨率的WalR DNA结合共有基序，并对由61个基因组成的直接调控子进行了系统表征。实验总体设计：在金黄色葡萄球菌（Staphylococcus aureus）中诱导表达经FLAG标签标记的细菌DNA结合蛋白，以开展染色质免疫沉淀测序（ChIP-seq）实验。