Vigna subterranea Transcriptome or Gene expression

Low temperature stress in a number of African countries, such as Botswana, at night can effect the growth and development of bambara groundnut, leading to losses in potential crop yield. Therefore, we made an attempt to identify and analyze the genes and gene modules associated with low temperature stress response in bambara groundnut using the cross-species microarray technique, as bambara groundnut has no microarray chip, coupled with network-based analysis. Overall design: Two plants of the bambara groundnut genotype 'S19-3' were grown in controlled environment growth rooms at Sutton Bonington Campus, University of Nottingham under a 12 hour photoperiod and at a constant temperature of 27°C. Plants were grown in soil columns containing a growing medium of 1 part John Innes 2 compost to 1 part sand, and were watered as required. A single, fully-expanded leaflet was sampled from each of the three plants growing at 27°C, snap frozen in liquid nitrogen and stored at -80°C. Plants were given a further 3 days at 27°C to recover from sampling before being moved to a controlled environment room at 23°C. On the fifth day at 23°C a single fully-expanded leaflet was sampled and stored, as described above. After 3 further days plants were moved to 18°C, and then five days later were sampled again.

在博茨瓦纳等多个非洲国家，夜间低温胁迫会影响班巴拉花生的生长发育，导致潜在作物产量损失。因此，鉴于班巴拉花生缺乏专用微阵列芯片，我们尝试结合跨物种微阵列技术与基于网络的分析方法，识别并解析班巴拉花生中与低温胁迫响应相关的基因及基因模块。
整体设计：两株班巴拉花生基因型'S19-3'植株于诺丁汉大学萨顿·波宁顿校区的可控环境生长室中培养，条件为12小时光周期及27℃恒温。从3株处于27℃环境的植株中各采集一片完全展开的小叶，迅速置于液氮中冷冻并于-80℃保存。植株在27℃环境下继续培养3天以恢复取样造成的影响，随后转移至23℃的可控环境室。在23℃环境下培养的第5天，按上述方法再次采集并保存一片完全展开的小叶。之后，植株再培养3天，转移至18℃环境，5天后再次取样。