Effect of Iomeprol on actin - Database

This dataset contains biophysical measurements designed to test the hypothesis that iodinated contrast agents can directly affect cytoskeletal proteins. Using purified actin, we examined how the clinically used agent Iomeprol influences actin structure, stability, and nucleotide binding. The data include thermal denaturation profiles, fluorescence‑based ATP‑binding assays, and polymerization measurements. Together, these experiments show that Iomeprol interacts with actin, induces detectable structural alterations, and reduces ATP affinity. Because actin is essential for cellular mechanics, these findings provide a molecular framework for interpreting how contrast agents may contribute to cellular stress in sensitive tissues.

本数据集包含旨在验证「碘化造影剂可直接作用于细胞骨架蛋白」这一假说的生物物理测量数据。本研究采用纯化的肌动蛋白（actin），考察了临床常用造影剂碘普罗胺（Iomeprol）对肌动蛋白结构、稳定性及核苷酸结合能力的影响。该数据集涵盖热变性曲线、基于荧光的ATP结合检测实验，以及聚合反应测量结果。综合上述实验结果表明，碘普罗胺可与肌动蛋白相互作用，诱导可检测到的结构改变，并降低其ATP结合亲和力。鉴于肌动蛋白对细胞力学功能至关重要，本研究结果为阐释造影剂如何在敏感组织中引发细胞应激提供了分子层面的理论框架。