SM009 - SARS infection of C57BL6, and PLAT knock-out mice.

This purpose of this experiment was to investigate the transcriptional differences between C57BL6, PLAT knock-out mice infected with SARS MA15 virus. Overview of Experiment: Groups of 20 week old C57BL6 and PLAT knock-out mice were infected with SARS MA15 virus. Infections were done at 10^5 PFU or time-matched mock infected. Time points were 4 and 7 d.p.i. There were 2-4 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

本实验旨在探究感染SARS MA15病毒的C57BL6小鼠与组织型纤溶酶原激活物（Plasminogen Activator Tissue-type，PLAT）敲除小鼠之间的转录组差异。
实验概况：选取20周龄的C57BL6小鼠与PLAT敲除小鼠，每类小鼠均分为两个亚组：分别以10^5 蚀斑形成单位（PFU）的剂量感染SARS MA15病毒，或接受时间匹配的模拟感染处理。实验采样时间点设为感染后第4天（4 d.p.i.）与感染后第7天（7 d.p.i.）。每个处理/时间点对应2-4只实验动物。采集肺组织样本，用于病毒载量检测、转录组及蛋白质组学分析。此外同步监测实验动物的体重丢失情况与存活状态。