Agrobacterium sp. 10MFCol1.1. Agrobacterium sp. 10MFCol1.1

Rhizosphere Grand Challenge Isolate Sequencing. The goal of this project is to comprehensively characterize the genomes of microorganisms found in the rhizosphere and endosphere of Arabidopsis thaliana. Organisms for study have been targeted based on their reproducible association with one or more Arabidopsis genotypes grown in natural soil. To obtain more complete genomes from the culturable fraction of the Arabidopsis microbiome, rhizosphere and endosphere samples were used to seed cultures on a variety of media. Cultures were screened by 16S rRNA sequencing and microbes of interest grown up and subjected to whole genome sequencing. Genomic sequences will be analyzed for genes participating in the plant-microbe symbiosis.

根际重大挑战分离株测序。本项目旨在全面解析拟南芥（Arabidopsis thaliana）根际（rhizosphere）与内生境（endosphere）中微生物的基因组特征。研究菌株的筛选依据为其与天然土壤中栽培的一种或多种拟南芥基因型存在可重复的关联关系。为从拟南芥微生物组的可培养组分中获取更完整的基因组，研究人员利用根际与内生境样本在多种培养基上接种培养。通过16S rRNA测序对培养物进行筛选，挑取目标微生物进行扩大培养并开展全基因组测序。后续将对基因组序列展开分析，以鉴定参与植物-微生物共生过程的基因。