Plasma PQQ and mtDNA/nuclear DNA Ratio Levels.

1Rats were fed an amino acid-based semi-purified diet either deficient in PQQ (PQQ−) or with PQQ added at 2 mg/kg diet (PQQ+). Rats initially fed PQQ− diets were also repleted with PQQ by i.p. injection (4.5 mg PQQ/Kg BW/24 hours X 3).2The superscripts *, **, *** represent significance relative to the PQQ+ group at p3The relative amounts of liver mitochondrial DNA (mtDNA) and nuclear DNA were measured by real-time PCR. The targeted genes were the nuclear cystic fibrosis and the mitochondrial nicotinamide adenine dinucleotide dehydrogenase-5 gene. When corresponding values for liver in each experiment are averaged, the liver values were significant at p

1. 大鼠被喂食基于氨基酸的半纯化饲料，分为两组：一组饲料缺乏吡咯喹啉醌（Pyrroloquinoline Quinone, PQQ），记作PQQ−组；另一组饲料中添加2 mg/kg剂量的PQQ，记作PQQ+组。对于初始喂食PQQ−饲料的大鼠，还通过腹腔注射方式进行PQQ补充，注射剂量为4.5 mg PQQ/千克体重，每24小时一次，连续3次。
2. 上标*、**、***代表相较于PQQ+组的统计学显著性，显著性判定阈值为p。
3. 采用实时荧光定量PCR（real-time PCR）检测肝脏线粒体DNA（mtDNA）与核DNA的相对含量。靶基因分别为核囊性纤维化基因以及线粒体烟酰胺腺嘌呤二核苷酸脱氢酶-5基因。将每次实验中肝脏的对应数值取平均后，肝脏相关检测值的统计学显著性为p。