ADAR1 suppression in human astrocytes

ADAR1 regulates the accumulation of endeognouse double-stranded RNA (dsRNA), a pro-inflammatory/innate immune activator. The purpose of this study was to measure the effects of ADAR1 suppression in human astrocytes and determine RNAs regulated by ADAR1. Primary human astrocytes were culture and transfected with a scramble or ADAR1 siRNA. RNA was isolated from these cells for total RNA-seq to analyze changes in gene/repetitive element expression and RNA editing. Overall design: Total RNA-seq on scramble siRNA and ADAR1 siRNA transfected primary human astrocytes

ADAR1可调控内源性双链RNA（double-stranded RNA, dsRNA）的积累，而该物质是一类促炎/天然免疫激活因子。本研究旨在评估ADAR1抑制在人星形胶质细胞中的作用效果，并鉴定受ADAR1调控的RNA分子。实验以原代人星形胶质细胞为材料，将其分别转染阴性对照小干扰RNA（scramble siRNA）与ADAR1靶向小干扰RNA（ADAR1 siRNA）。随后提取上述细胞的总RNA，开展总RNA测序（total RNA-seq），以分析基因与重复元件的表达变化及RNA编辑情况。实验整体设计：对转染阴性对照siRNA与ADAR1 siRNA的原代人星形胶质细胞进行总RNA测序。