4T1 cultured mouse mammary tumor cell RNA-seq gene expression profiling: impact of activated cyclophosphamide versus interferon-beta treatment, and the role of interferon signaling in cyclophosphamide responses

RNA-seq analysis of polyA-selected RNA isolated from cultured 4T1 mouse breast cancer cells, with or without treatment with activated cyclophosphamide (4HC, 4-hydroperoxy-cyclophosphamide), interferon-beta, and antibody to the type-I interferon receptor IFNAR1. These RNA samples were part of a study where we investigated the role of type-I interferon signaling in therapeutic responses to cyclophosphamide in mouse models of breast cancer [Vergato et al, Canc Res Communic]. RNA-seq libraries were prepared from cultured tumor cell-extracted RNA obtained from n=2-3 independent cultures for each condition tested.

本数据集针对从培养的4T1小鼠乳腺癌细胞中分离的聚腺苷酸富集RNA（polyA-selected RNA）开展RNA测序（RNA-seq）分析，设置了未处理对照组与如下处理组：分别经活化环磷酰胺（activated cyclophosphamide，4HC，4-hydroperoxy-cyclophosphamide）、β干扰素（interferon-beta）以及I型干扰素受体IFNAR1抗体处理。上述RNA样本来自一项探究I型干扰素信号通路在小鼠乳腺癌模型中环磷酰胺治疗应答中作用的研究[Vergato等，Canc Res Communic]。针对每个测试条件，我们从n=2-3个独立培养的肿瘤细胞提取的RNA中制备了RNA测序文库。