Data Accompanying: Berger, Alvarado, and Kiss (2019)

Cytoplasmic capping is an understudied epitranscriptomic RNA modification that could have large ramifications on gene expression. Simply, cytoplasmic capping returns a N7-methylguanosine cap onto the 5' end of an uncapped RNA. In the paper linked to this work, we detect uncapped and truncated 5' mRNA ends in cells were cytoplasmic capping is normal in addition to cells where it has been blocked. The majority of these uncapped ends map to previously identified cap sites. Further, we show data which hint that recapping may be driven by sequences of the RNAs themselves. Together, these data support earlier studies andhint that alternative splicing may aid in selecting or targeting mRNAs for cytoplasmic capping. The data posted here are supporting data (including gels and/or blots showing additional sample replicates for our experiments, additional cloned sequences information, etc) that could not be published with the paper due to page constraints and/or standard conventions (like only showing 1 replicate in a publication).

细胞质加帽（cytoplasmic capping）是一种尚未得到充分研究的表观转录组学（epitranscriptomic）RNA修饰，其可能对基因表达产生深远影响。简言之，细胞质加帽是将N7-甲基鸟苷帽结构（N7-methylguanosine cap）添加至未带帽RNA的5'端。
在本研究关联的论文中，我们分别在细胞质加帽功能正常和被阻断的细胞中，检测到了未带帽及截短的5'端mRNA末端。这些未带帽末端大多定位至此前已鉴定的帽位点。此外，我们的研究数据提示，重新加帽过程可能由RNA自身的序列所驱动。综上，这些数据既支持了此前的相关研究，同时也提示可变剪接（alternative splicing）可能参与选择或靶向用于细胞质加帽的mRNA。
本数据集所提供的均为补充数据，包括实验的额外样本重复结果（如凝胶电泳与免疫印迹的额外重复样本数据）、额外的克隆序列信息等。由于版面限制及学术出版的常规规范（例如期刊通常仅展示1次重复实验结果），此类数据未能随主论文一同刊发。