Candidatus Endomicrobiellum trichonymphae RefSeq Genome. Candidatus Endomicrobiellum trichonymphae

uncultured Termite group 1 bacterium phylotype Rs-D17. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of Trichonympha agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host Trichonympha agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.

未培养的白蚁类群1细菌系统型Rs-D17。实验所用食木白蚁为日本散白蚁（Reticulitermes speratus）（等翅目（Isoptera）；鼻白蚁科（Rhinotermitidae）），采集自日本埼玉县。研究人员从一只工白蚁体内剥离完整肠道，利用显微操作仪对肠道内分解纤维素的原生生物敏捷披发虫（Trichonympha agilis）（副基纲（Parabasalia）；披发虫目（Trichonymphida））的单个细胞进行物理分离。为获取该披发虫的胞内共生菌——属于候选类群白蚁类群1、暂未培养的细菌系统型Rs-D17，研究人员在缓冲液中裂解宿主披发虫细胞，收集溢出的细菌细胞，再通过玻璃毛细管完成富集。收集得到的细胞直接进行恒温全基因组扩增。通过16S核糖体RNA基因及其相邻内转录间隔区（Internal Transcribed Spacer, ITS）的克隆分析验证纯度后，研究人员使用ABI 3730毛细管测序仪与GS20测序系统完成了该细菌的全基因组序列测定。