Bee-collected pollen Targeted loci environmental. pollen metagenome

We extracted DNA from honey bee-collected pollen and co-amplified the ITS2, rbcL and matK loci using plant-specific primers. Amplicon libraries were then sequenced on the Illumina MiSeq platform.

我们从蜜蜂采集的花粉中提取脱氧核糖核酸（DNA），并使用植物特异性引物对ITS2、rbcL以及matK基因座进行共扩增。随后在Illumina MiSeq测序平台上对扩增子文库开展测序。