Research data supporting “A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli" (PhD thesis)

The supplemental file contains a list of publications used to conduct a systematic literature review for the thesis titled “A Systematic Method for Enhanced Expression of Alcohol Dehydrogenases in Escherichia coli”. Publications for this review were sourced from NCBI PubMed and WoS databases, in addition to Google Scholar. Relevant articles published between 1 January 2010 and 13 April 2022 were selected. Search terms included those for Escherichia coli (including the terms ‘Escherichia coli’ and ‘E. coli’), ‘recombinant’, ‘enzyme’, and ‘inclusion bodies’, generated a total of 791 results in PubMed, 232 results in WoS and 40.1 K search results in Google Scholar. These papers were manually curated within the search results for PubMed and WoS. Logical operators were used to remove irrelevant search results from the large number of results returned in Google Scholar specifically leaving 780 results. The literature search for this review was limited to those studies that explicitly reported: (i) the native prokaryotic source of the enzyme of interest, (ii) gene sequence and/or identifier, (iii) the E. coli expression strain, (iv) the expression vector backbone, and (v) expression conditions (i.e. expression temperature, inducer concentration, cell density). In total 139 publications that expressed 146 enzymes were selected between 2010 and 2022; some publications highlighted multiple enzymes leading to a higher number of enzymes compared to publications.

本补充文件包含用于开展题为《大肠杆菌（Escherichia coli）乙醇脱氢酶（Alcohol Dehydrogenases）增强表达的系统化方法》的学位论文所使用的系统综述（systematic literature review）文献列表。本次综述的文献来源除Google Scholar外，涵盖NCBI PubMed数据库与WoS数据库。筛选范围限定为2010年1月1日至2022年4月13日期间发表的相关文献。检索词涵盖针对大肠杆菌（含“Escherichia coli”与“E. coli”表述）、重组（recombinant）、酶（enzyme）以及包涵体（inclusion bodies）的检索式，最终在PubMed中获得791条检索结果，WoS中获得232条，Google Scholar中获得40.1千条检索结果。研究人员对PubMed与WoS的检索结果进行了人工筛选整理；针对Google Scholar返回的海量检索结果，通过逻辑运算符剔除无关文献，最终保留780条结果。本次综述的文献检索限定于明确报告以下内容的研究：(i) 目标酶的天然原核来源；(ii) 基因序列及/或基因标识符；(iii) 大肠杆菌表达菌株；(iv) 表达载体骨架；(v) 表达条件（即表达温度、诱导剂浓度、细胞密度）。最终在2010年至2022年间筛选出139篇文献，共涉及146种酶；由于部分文献报道了多种酶，因此酶的总数多于文献数量。