Methyloversatilis sp. NSM2 Genome sequencing and assembly. Methyloversatilis sp. NSM2

This bacterium was isolated from the aerobic bioreactor of a wastewater treatment plant (TRARGISA - Waste and Wastewater Treatment System of Girona S.A.), specifically from the point where it interfaces with the recirculation stream from the anaerobic bioreactor. The mixed liquor sludge sample was used as inoculum for methanotrophic enrichment, using a modified NMS medium (as described in doi.org/10.1016/j.bej.2018.01.003) with methane as the sole carbon source (20% v/v). After a 10-month enrichment period, samples were cultured on mod-NMS medium agar plates in a methane-enriched atmosphere. Subsequently, single colonies were isolated and purified through successive plating.

该细菌分离自某污水处理厂的好氧生物反应器（TRARGISA——赫罗纳废物与污水处理系统股份公司），具体采样点位位于该反应器与厌氧生物反应器回流液流的接口处。以采集的混合液污泥样品作为接种物开展甲烷氧化菌富集培养，培养体系采用改良NMS培养基（modified NMS medium，详见doi.org/10.1016/j.bej.2018.01.003），以甲烷作为唯一碳源，其体积占比为20%。经过10个月的富集培养周期后，将样品接种于改良NMS培养基琼脂平板，置于富甲烷气氛中进行培养。随后通过连续平板划线操作分离得到单菌落并完成纯化。