Transmittance and bidirectional reflectance changes induced by blue light in Arabidopsis wild type and chloroplast movement mutants

Reflectance single polarizer setup: Time courses of changes in red-light bidirectional reflectance induced by 1 h irradiation with low (0.1 µmol·m^-2·s^-1) or high (100 µmol·m^-2·s^-1) blue light of the adaxial surface of rosette leaves of 5-week-old <i>Arabidopsis</i> plants. Measurements were recorded for wild-type (WT), <i>glabra1, phot1</i>,<i> phot2</i>, <i>phot1phot2,</i> and <i>jac1 </i>mutants. The angle of incidence of the measuring and actinic beams was 40° or 55°. The detector was positioned at the angle of 0° (the detector normal parallel to the leaf normal) or either -40° (when incidence at 40°) or -55° (when incidence at 55°). The transmission axis of the polarizer was either parallel (<i>P</i>) or perpendicular (<i>S</i>) to the plane of incidence.Reflectance two polarizer setup: Time courses of the polarized red-light bidirectional reflectance induced by 1 h irradiation with high blue light (100 µmol·m^-2·s^-1) of the adaxial surface of rosette leaves of 5-week-old wild-type and <i>phot1phot2</i> <i>Arabidopsis</i> plants. The blue actinic and red measuring beams impinged the adaxial leaf surface at the angle of 55°. For measurements of diffuse reflectance, the detector was positioned at 0° to the leaf’s normal. For measurements of combined specular and diffuse reflectance, the detector was positioned at -55°. Four possible combinations of the orientation (<i>P/S</i>) of the polarizers in front of the measuring light source and the detector were examinedBinary files can be opened with BeamJ (https://github.com/pawelHerm/beamJ/tree/master/BeamJ), while the CSV files can be opened with a text editor or MS Excel.

反射单偏振片装置：5周龄拟南芥（*Arabidopsis*）植株莲座叶近轴面经低强度（0.1 µmol·m⁻²·s⁻¹）或高强度（100 µmol·m⁻²·s⁻¹）蓝光照射1小时后，红光双向反射率的时间变化过程。测试样本涵盖野生型（WT）、*glabra1*、*phot1*、*phot2*、*phot1phot2*及*jac1*突变体。测量光束与光化光束的入射角度为40°或55°。探测器的放置角度分为两类：一类为0°（探测器法线与叶片法线平行），另一类分别对应入射角度40°时的-40°，以及入射角度55°时的-55°。偏振片的透射轴与入射平面的相对取向为平行（P）或垂直（S）。

反射双偏振片装置：5周龄野生型及*phot1phot2*拟南芥植株莲座叶近轴面经高强度蓝光（100 µmol·m⁻²·s⁻¹）照射1小时后，偏振红光双向反射率的时间变化过程。蓝光光化光束与红光测量光束以55°入射角度照射叶片近轴面。其中，漫反射测量时探测器放置于与叶片法线成0°的位置；镜面反射与漫反射组合测量时，探测器放置于-55°的位置。本实验考察了测量光源前偏振片与探测器偏振片四种可能的取向组合（P/S）。

二进制文件可通过BeamJ（https://github.com/pawelHerm/beamJ/tree/master/BeamJ）打开，CSV文件可通过文本编辑器或MS Excel打开。