Differential innate immune signaling in macrophages by wild type vaccinia virus and mutant virus deleting A26 protein

We infected bone marrow derived macrophages (BMDM) with wild type vaccinia virus Western Reserve (WT-WR) and mutant virus deleting A26 protein (WRΔA26) and analyzed transcriptional profiling of cellular genes at 1, 2, 4 and 8 hours post-infection times (p.i.). Two-condition experiment, WT-WR VS WRΔA26 infected BMDM cells at 4 time points. Biological replicates: 3 infection replicates.

我们使用野生型痘苗病毒韦斯顿储备株（WT-WR）以及缺失A26蛋白的突变病毒（WRΔA26）感染骨髓源巨噬细胞（BMDM），并在感染后1、2、4、8小时（p.i.）对细胞基因的转录组谱展开分析。本实验为双条件实验，在4个时间点下分别检测WT-WR感染与WRΔA26感染的BMDM细胞。本次实验的生物学重复设置为3次感染重复。