Additional file 11: of Microtubule polyglutamylation and acetylation drive microtubule dynamics critical for platelet formation
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Movie S8-S10. MT acetylation level must be controlled for efficient PPLL formation in D723H cell spreading on fibrinogen. D723H cells spreading on fibrinogen treated with carrier, TSA or TBSA as indicated and imaged every 5Â min with an inverted Leica DMIRE2 microscope equipped with a Leica 20X N PLAN L 0.4 LMC and driven by metamorph software. (ZIP 2318 kb)
补充视频S8-S10。在纤维蛋白原表面铺展的D723H细胞中，若要高效形成PPLL（Podosome-Pseudopod Like Structure），需对微管（Microtubule, MT）乙酰化水平进行精准调控。实验中，将铺展于纤维蛋白原上的D723H细胞按实验指示分别以载体对照、TSA（Trichostatin A，曲古抑菌素A）或TBSA处理，随后借助配备Leica 20× N PLAN L 0.4 LMC物镜的倒置Leica DMIRE2显微镜，由MetaMorph软件驱动，每5分钟采集一次成像画面。（压缩包大小：2318 KB）
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2018-10-19
