Evaluation of antimicrobial action of silver composite microspheres based on styrene-divinylbenzene copolymer

Abstract This article reports the evaluation of the antimicrobial activity of a silver composite based on sulfonic resin. The antimicrobial action of the composite was evaluated against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus through plate, batch and colunm experiments. In batch studies, the efficiency of the composite was evaluated as a function of composite mass, bacterial concentration and contact time. We also developed a method to evaluate the antimicrobial activity of this composite using column tests. The antimicrobial activity of the composite was similar against the three bacteria in halo inhibition and batch experiments. The antibacterial activity was 100% against all bacteria above 0.20 g of composite and for all concentrations of bacteria studied. Column studies showed that the composite (1 g) had 100% action against 48 cm3 of S. aureus and 55 cm3 of E. coli and P. aeruginosa suspensions (105 cells mL-1, 50 cm3 min-1).

摘要 本研究报道了一款基于磺酸树脂（sulfonic resin）的银基复合材料的抗菌活性评价工作。本研究通过平板实验、批量实验与柱实验，评价了该复合材料对大肠埃希氏菌（Escherichia coli）、铜绿假单胞菌（Pseudomonas aeruginosa）以及金黄色葡萄球菌（Staphylococcus aureus）的抗菌作用。在批量实验中，本研究以复合材料投加量、细菌浓度与接触时间为变量，评价了该复合材料的抗菌效能。此外，本研究还开发了一种利用柱实验评价该复合材料抗菌活性的方法。在抑菌圈实验与批量实验中，该复合材料对三种受试菌的抗菌活性表现相近。当复合材料投加量高于0.20 g时，本研究所涉及的所有细菌浓度下，该复合材料对三种细菌均表现出100%的抗菌活性。柱实验结果显示，投加1 g该复合材料时，可对48 cm³的金黄色葡萄球菌（S. aureus）悬液以及55 cm³的大肠埃希氏菌（E. coli）、铜绿假单胞菌（P. aeruginosa）悬液（浓度为10^5 个·mL⁻¹，流速为50 cm³·min⁻¹）实现100%的抗菌作用。