Expression data from human lung cancer cell line NCI-H292. Expression data from human lung cancer cell line NCI-H292

CT45 family is abnormally overexpressed in various types of cancer. However, the molecular mechanisms of the oncogenic CT45 to trigger carcinogenesis and tumor malignant progression are largely enigmatic. We previously reported that CT45A1 functions as a proto-oncogene to promote the transcription in the human breast cancer cells, thereby escalating tumorigenesis and cancer metastasis, However, the mechanism of CT45A1-mediated concurrent overexpression of the multiple oncogenic genes in cancer is unclear. We used microarrays to study the role and mechanism of CT45A1 in gene transcriptional regulation in human lung cancer. Overall design: Three replicates of NCI-H292_CT45A1-overexpression VS three replicates of NCI-H292_CT45A1-negative

CT45家族在多种癌症中呈现异常高表达。然而，致癌性CT45家族成员诱发癌变及肿瘤恶性进展的分子机制在很大程度上仍不明晰。我们此前曾报道，CT45A1作为原癌基因，可促进人乳腺癌细胞的基因转录，进而促进肿瘤发生与癌症转移。然而，CT45A1介导癌症中多种致癌基因协同高表达的具体机制仍未明确。本研究采用微阵列（microarrays）技术，探究CT45A1在人肺癌细胞中的基因转录调控作用与机制。实验整体设计：设置NCI-H292_CT45A1过表达组与NCI-H292_CT45A1阴性对照组，每组均设3次生物学重复。